Products
HemaFus PML-RARA (bcr1, bcr2, and bcr3) Qualitative Detection Kit (PCR-Fluorescent Probing)
Cat. No.:
J0366
Spec:
24 Tests/Kit
Product description
Parameters
Background
Acute promyelocytic leukemia (APL) is a distinct subtype of AML characterized by specific chromosomal translocation (t15;17)(q22;q12) in the vast majority of patients. This leads to the formation of the PML-RARA fusion gene (Figure 1), and its protein product causes cell differentiation arrest and apoptosis deficiency, which is the main molecular mechanism of APL development.
Figure 1
PML/RARA fusion gene is heterogeneous. Since PML gene recombination occurs in different breakpoint cluster regions, three isoforms have been identified (Figure 2): (1) Long isoform (L; bcr-1) caused by a breakpoint in PML resulting in fusion of RARA (exon 3) with PML (exon 6); (2) Variant isoform (V; bcr-2). Caused by a breakpoint in PML resulting in fusion of RARA (exon 3) with PML (exon 6); (3) Short isoform (S; bcr-3), caused by a breakpoint in PML resulting in fusion of RARα (exon 3) with PML (exon 3). The PML/RARα fusion gene is highly correlated with M3, and its detection results are helpful for the diagnosis of M3.
Figure 2
Detection Process
In this method, RNA is isolated and purified from leukocytes in peripheral blood or bone marrow samples, and cDNA is obtained by reverse transcription to detect bcr1, bcr2, and bcr3 types of PML-RARA fusion gene by real-time Q-PCR. PML-RARA fusion is shown in Figure 3.
Figure 3
Quantitative Kit Detection Results
Specimen Requirements
Peripheral blood or bone marrow (3–5 mL) is collected in the EDTA tube and stored at 2–8 °C for not more than 5 days. RNA extraction is performed within 5 days.
References
[1] NCCN Clinical Practice Guidelines in Oncology: Acute Myeloid Leukemia (version 3.2017)
[2] Leukemia,2003,17(12):2318-2357
[3] Leukemia(1999)13,1901-1928.
Ordering Information
| Cat. No. | Product | Strength |
|
T0305 |
HemaFus PML-RARa(bcr1) Quantitative Detection Kit
|
24 Tests/Kit |
|
T0306 |
HemaFus PML-RARa(bcr2) Quantitative Detection Kit
|
24 Tests/Kit |
|
T0307 |
HemaFus PML-RARa(bcr3) Quantitative Detection Kit
|
24 Tests/Kit |
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