Products
HemaFus PML-RARA (bcr1) Fusion Gene Quantitative Detection Kit (PCR-Fluorescent Probing)
Background
Acute promyelocytic leukemia (APL) is a distinct subtype of AML characterized by specific chromosomal translocation (t15;17)(q22;q12) in the vast majority of patients. This leads to the formation of the PML-RARA fusion gene (Figure 1), and its protein product causes cell differentiation arrest and apoptosis deficiency, which is the main molecular mechanism of APL development.
Figure 1
PML/RARA fusion gene is heterogeneous. Since PML gene recombination occurs in different breakpoint cluster regions, three isoforms have been identified (Figure 2): (1) Long isoform (L; Bcr-1), caused by a breakpoint in PML resulting in fusion of RARA (exon 3) with PML (exon 6); (2) Variant isoform (V; Bcr-2), caused by a breakpoint in PML resulting in fusion of RARA (exon 3) with PML (exon 6); (3) Short isoform (S; Bcr-3), caused by a breakpoint in PML resulting in fusion of RARA (exon 3) with PML (exon 3). The PML/RARA fusion gene is highly correlated with AML-M3, and its detection results are helpful for the diagnosis of AML-M3.
Figure 2
Features
Simple operation and less time consumption; reliable results and intuitive interpretation; high sensitivity and sample saving.
Detection Process
The bcr1 subtype of PML-RARA fusion gene is quantitatively detected by fluorescence quantitative PCR.
Ordering Information
Cat. No. | Product | Strength |
J0366 |
HemaFus PML-RARA (bcr1, bcr2, and bcr3) Qualitative Detection Kit
|
24 Tests/Kit |
T0305 |
HemaFus PML-RARA (bcr1) Fusion Gene Quantitative Detection Kit
|
24 Tests/Kit |
T0306 |
HemaFus PML-RARA (bcr2) Fusion Gene Quantitative Detection Kit
|
24 Tests/Kit |
T0307 |
HemaFus PML-RARA (bcr3) Fusion Gene Quantitative Detection Kit
|
24 Tests/Kit |