Products
HemaFus Human 60-Fusion Gene Screening Kit (PCR-Fluorescent Probing)
Cat. No.:
T0690
Spec:
24 Tests/Kit
Product description
Parameters
Background
The fusion gene, formed by chromosomal translocation and rearrangement, is the result of both genetic and environmental factors. In the process of leukemia diagnosis and typing, fusion genes play a very important role, which can be used for diagnosis and typing for special types of leukemia. Moreover, fusion genes are also major molecular markers for prognostic stratification, minimal residual disease (MRD) monitoring, and targeted therapy in leukemia patients.
Detection Process
In this method, RNA is isolated and purified from leukocytes in peripheral blood or bone marrow samples, and cDNA is obtained by reverse transcription and then a total of 60 fusion genes, including 241 subtypes, will be detected through 16 wells using multiple real-time fluorescence quantitative PCR in conjunction with Taqman probes. Covers AML, ALL, CML, other mixed cell type leukemias, as well as leukemia related fusion genes that have not been clearly classified. It helps to determine the type of fusion genes, thereby assisting in clinical diagnosis, selection of drug for clinical use, and evaluation of disease prognosis for patients with leukemia.
Display of reagent kit results
Figure 1 shows that the reagent kit successfully detects the CBFB:: MYH11 fusion gene;
Figure 2 shows that the reagent kit successfully detects the ETV6:: PDGFRB fusion gene
Gene detection list
|
Fusion gene (60 types) |
Fusion type |
Number of fusion forms (241 types) |
|
BCR::ABL1 P190 |
e1a2 |
1 |
|
BCR::ABL1 P210 |
e13a2/e14a2 |
2 |
|
BCR::ABL1 P230 |
e19a2 |
1 |
|
BCR: ABL1, other fusion |
e1a3/e6a2/e6a3/e8a2/e8a3/e13a3/e14a3/e18a2/e18a3/e19a3 |
10 |
|
BCR::FGFR1 |
e4a10 |
1 |
|
CBFA2T3::GLS2 |
e9a3/e10a3/e11a4/e12a2 |
4 |
|
CBFB::MYH11 |
e5a33/e5a30/e5a29 |
3 |
|
DEK::NUP214(DEK-CAN) |
e8a17/e8a18 |
2 |
|
EML1::ABL1 |
e18a2 |
1 |
|
ETV6::ABL1 |
e4a2/e5a2 |
2 |
|
ETV6::PDGFRA |
e6a12 |
1 |
|
ETV6::RUNX1 |
e4a3/e5a3/e5a3/e5a4 |
4 |
|
ETV6::PDGFRB |
e4a9/e4a10/e4a11/e5a4 |
4 |
|
FIP1L1::PDGFRA |
e9a12/e11a12/e13a12 |
3 |
|
FUS::ERG |
e6a10/e7a10/e7a8/e8a8 |
4 |
|
KAT6A::CREBBP |
e16a2/e17a2/e16a3/e16a4/e15a4/e15a5 |
6 |
|
KMT2A::AFDN(MLL-AF6) |
e7a2/e8a2/e9a2/e10a2 |
4 |
|
KMT2A::AFF1(MLL-AF4) |
e7a4/e7a5/e7a6/e7a9/e7a11/e8a4/e8a5/e8a6/e8a9/e8a11/e9a4/e9a5/e9a6/e9a9/e9a11/e10a4/e10a5/e10a6/e10a9/e10a11 |
20 |
|
KMT2A::CREBBP(MLL-CREBBP) |
e7a3/e8a3/e9a3/e10a3/e7a16/e8a16/e9a16/e10a16 |
8 |
|
KMT2A::ELL(MLL-ELL) |
e7a2/e8a2/e9a2/e10a2/e7a6/e8a6/e9a6/e10a6 |
8 |
|
KMT2A::EPS15(MLL-AF1P) |
e7a2/e8a2/e9a2/e10a2/e7a6/e8a6/e9a6/e10a6/e7a9/e8a9/e9a9/e10a9/e7a10/e8a10/e9a10/e10a10 |
16 |
|
KMT2A::FOXO4(MLL-AFX1) |
e7a2/e8a2/e9a2/e10a2 |
4 |
|
KMT2A::MLLT1(MLL-ENL) |
e7a2/e7a4/e7a6/e7a7/e8a2/e8a4/e8a6/e8a7/e9a2/e9a4/e9a6/e9a7/e10a2/e10a4/e10a6/e10a7 |
16 |
|
KMT2A::MLLT10(MLL-AF10) |
e7a6/e8a6/e9a6/e10a6/e7a9/e8a9/e9a9/e10a9/e7a10/e8a10/e9a10/e10a10/e7a11/e8a11/e9a11/e10a11/e7a12/e8a12/e9a12/e10a12/e7a15/e8a15/e9a15/e10a15/e7a16/e8a16/e9a16/e10a16/ |
28 |
|
KMT2A::MLLT11(MLL-AF1Q) |
e7a2/e8a2/e9a2/e10a2 |
4 |
|
KMT2A::MLLT3(MLL-AF9) |
e7a5/e7a6/e7a9/e7a10/e8a5/e8a6/e8a9/e8a10/e9a5/e9a6/e9a9/e9a10/e10a5/e10a6/e10a9/e10a10 |
16 |
|
KMT2A::MLLT6(MLL-AF17) |
e7a9/e7a11/e8a9/e8a11/e9a9/e9a11/e10a9/e10a11 |
8 |
|
MNX1::ETV6 |
e1a2/e1a3 |
2 |
|
NPM1::ALK |
e4a20 |
1 |
|
NPM1::MLF1（ROX2) |
e6a2 |
1 |
|
NPM1::RARA |
e4a3 |
1 |
|
NUP98 related fusions(NUP98:: HOxA9/NUP98:: HOxD13/NUP98:: PMX1/NUP98:: HOxA11/NUP98:: HOxA13/NUP98:: NSD1/NUP98:: HOxC11/NUP98: KDM5A/NUP98:: RARG/NUP98: TOP1) |
e12a1/e12a2/e12a2/e12a2/e12a2/e12a7/e12a2/e13a27/e12a7/e12a9 |
10 |
|
PICALM::MLLT10(CALM-AF10) |
e17a6/e17a9/e17a10/e17a11/e17a12/e17a15/e17a16/e19a6/e19a9/e19a10/e19a11/e19a12/e19a15/e19a16 |
14 |
|
PML::RARA BCR1 |
e6a3 |
1 |
|
PML::RARA BCR2 |
/ |
1 |
|
PML::RARA BCR3 |
e3a3 |
1 |
|
RARA related fusion |
e15a3/e22a3/e15a3/e3a3/e11a3/e5a3 |
6 |
|
RBM15::MRTFA(RBM15-MKL1) |
e1a5 |
1 |
|
RPN::EVI1 |
e1a2/e1a3 |
2 |
|
RUNX1::CBFA2T3(RUNX1-MTG16) |
e6a2/e6a4 |
2 |
|
RUNX1::EVI1(AML-EVI1) |
e6a3 |
1 |
|
RUNX1::MDS1(AML-MDS1) |
e6a2 |
1 |
|
RUNX1::MECOM(AML-MDS1-EVI1) |
e6a2a3 |
1 |
|
RUNX1::RPL22(RUNX1-EAP) |
e6a2 |
1 |
|
RUNX1::RUNX1T1（AML1-ETO） |
e6a2 |
1 |
|
SET::NUP214(SET-CAN) |
e7a17/e7a18 |
2 |
|
STIL::TAL1(STL-TAL1) |
e1a3/e1a4/e2a3 |
3 |
|
TCF3::HLF |
e14a4/e15a4/e16a4 |
3 |
|
TCF3::PBX1 |
e16a3 |
1 |
|
ZBTB16::RARA(PLZF-RARA) |
e3a3/e4a3 |
2 |
|
ZMYM2::FGFR1(ZNF198-FGFR1) |
e17a10 |
1 |
References
[1] Nature Communications (2023)14:1739.
[2] International Journal of Hematology (2023) 117:173-181.
[3] Leukemia(1999)13,1901-1928.
Ordering Information
|
Cat. No.
|
Product
|
Strength
|
|
T0690 |
HemaFus Human 60-Fusion Gene Screening Kit
|
24 Tests/Kit |
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